Recently, corresponding to the lengthening of our life span, obesity which predisposes a person to diabetes mellitus have gradually been increased as well as the number- of diabetes. Therefore, diabetes mellitus has been one of the most important problems in modern medical science.
This study was conducted to ellucidate the effects of nicotinamide, nicotinic acid, L-cysteine, tolbutamide, diazoxide, D-glucose, D-mannose, 3-0-methyl-D-glucose and 2-deoxy-D-glucose on streptozotocin-induced diabetic mice.
All drugs except streptozotocin dissolved in normal saline were injected intraperitoneally. Streptozotocin dissolved in acidified citrate buffer (pH4.5) was immediately injected intraperitoneally. Serum blood glucose level was determined 48 hours later.
The results obtained from this study were as follows:
1. Serum glucose levels were elevated to 2 hours after administration of streptozotocin and decreased to 10 hours after administration of streptozotocin. Serum glucose levels were elevated from 10 hours after administration of streptozotocin and maximum serum glucose level was achieved in 24 hours after ad-ministration of streptozotocin (430 31 mg/dl) and maintained the elevated state.
2. Nicotinamide protected almost completely against streptozotocin-induced hyperglycemia (92% inhibition of serum glucose levels in streptozotocin ad-ministered group).
3. 2 -deoxy-D -glucose protected partially (67% inhibition of serum glucose levels in streptozotocin administred group) and 3-0-methyl-D-glucose protected almost completely against streptozotocin-induced hyperglycemia (97% inhibition of serum glucose levels in streptozotocin administered group).
4. 3-0-methyl-D-glucose and 2-deoxy-D-glucose were additive in the portection against streptozotocin-induced hyperglycemia.
5. D-glucose, D-mannose, tolbutamide, diazoxide, L-cysteine and nicotinic acid did not protected the streptozotoin -induced hyperglycemia.
|